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A. Confocal projection of the head of DAF-28::mCherry; pdaf-7 ::GFP animal, with both ASI neurons visible. DAF-28::mCherry protein (red) localizes exclusively to axons of the ASI and other neurons, and is excluded from dendrites. Green: GFP expressed from a pdaf-7 ::GFP; rol-6 array, used to visualize the ASI. The third dendrite in A is from another animal. A’ shows the axonal area. B. 3D confocal projections of axonal area of the animal in Figure 2B,C , showing both left and right neurons together ( left panel ) and separately; DAF-28::mCherry stereotypically accumulates in synapse-rich areas (ovals in A’ and B). Cell bodies of the ASI, ASJ, ASK, and likely Bag neurons are indicated. C. DAF-28::mCherry is secreted from mammalian cells. <t>Dot</t> <t>blot</t> of DAF-28::mCherry protein in media supernatant from transiently transfected HEK293T cells. The presence of DAF-28::mCherry protein in the media decreases dramatically following 24 hour incubation with 2.5 µg/mL BFA, indicating active vesicle-mediated release. Cell-number equivalents for the amount of media applied in each well are indicated at the bottom. D. Video of a section of the VNC (arrowheads) in the tail area, imaged from the ventral side. DAF-28::mCherry moves in bright puncta. A corresponding video file is Sup. Video 1 . Scale bar for B = 5μm, for D = 20μm.
Dot Blot Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dot blot apparatus/product/Bio-Rad
Average 99 stars, based on 1 article reviews
dot blot apparatus - by Bioz Stars, 2026-06
99/100 stars
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96 well dot blot apparatus  (Bio-Rad)
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Bio-Rad 96 well dot blot apparatus
A. Confocal projection of the head of DAF-28::mCherry; pdaf-7 ::GFP animal, with both ASI neurons visible. DAF-28::mCherry protein (red) localizes exclusively to axons of the ASI and other neurons, and is excluded from dendrites. Green: GFP expressed from a pdaf-7 ::GFP; rol-6 array, used to visualize the ASI. The third dendrite in A is from another animal. A’ shows the axonal area. B. 3D confocal projections of axonal area of the animal in Figure 2B,C , showing both left and right neurons together ( left panel ) and separately; DAF-28::mCherry stereotypically accumulates in synapse-rich areas (ovals in A’ and B). Cell bodies of the ASI, ASJ, ASK, and likely Bag neurons are indicated. C. DAF-28::mCherry is secreted from mammalian cells. <t>Dot</t> <t>blot</t> of DAF-28::mCherry protein in media supernatant from transiently transfected HEK293T cells. The presence of DAF-28::mCherry protein in the media decreases dramatically following 24 hour incubation with 2.5 µg/mL BFA, indicating active vesicle-mediated release. Cell-number equivalents for the amount of media applied in each well are indicated at the bottom. D. Video of a section of the VNC (arrowheads) in the tail area, imaged from the ventral side. DAF-28::mCherry moves in bright puncta. A corresponding video file is Sup. Video 1 . Scale bar for B = 5μm, for D = 20μm.
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https://www.bioz.com/result/96 well dot blot apparatus/product/Bio-Rad
Average 99 stars, based on 1 article reviews
96 well dot blot apparatus - by Bioz Stars, 2026-06
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dot blot vacuum apparatus  (Bio-Rad)
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A. Confocal projection of the head of DAF-28::mCherry; pdaf-7 ::GFP animal, with both ASI neurons visible. DAF-28::mCherry protein (red) localizes exclusively to axons of the ASI and other neurons, and is excluded from dendrites. Green: GFP expressed from a pdaf-7 ::GFP; rol-6 array, used to visualize the ASI. The third dendrite in A is from another animal. A’ shows the axonal area. B. 3D confocal projections of axonal area of the animal in Figure 2B,C , showing both left and right neurons together ( left panel ) and separately; DAF-28::mCherry stereotypically accumulates in synapse-rich areas (ovals in A’ and B). Cell bodies of the ASI, ASJ, ASK, and likely Bag neurons are indicated. C. DAF-28::mCherry is secreted from mammalian cells. <t>Dot</t> <t>blot</t> of DAF-28::mCherry protein in media supernatant from transiently transfected HEK293T cells. The presence of DAF-28::mCherry protein in the media decreases dramatically following 24 hour incubation with 2.5 µg/mL BFA, indicating active vesicle-mediated release. Cell-number equivalents for the amount of media applied in each well are indicated at the bottom. D. Video of a section of the VNC (arrowheads) in the tail area, imaged from the ventral side. DAF-28::mCherry moves in bright puncta. A corresponding video file is Sup. Video 1 . Scale bar for B = 5μm, for D = 20μm.
Dot Blot Vacuum Apparatus, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dot blot vacuum apparatus/product/Bio-Rad
Average 99 stars, based on 1 article reviews
dot blot vacuum apparatus - by Bioz Stars, 2026-06
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A. Confocal projection of the head of DAF-28::mCherry; pdaf-7 ::GFP animal, with both ASI neurons visible. DAF-28::mCherry protein (red) localizes exclusively to axons of the ASI and other neurons, and is excluded from dendrites. Green: GFP expressed from a pdaf-7 ::GFP; rol-6 array, used to visualize the ASI. The third dendrite in A is from another animal. A’ shows the axonal area. B. 3D confocal projections of axonal area of the animal in Figure 2B,C , showing both left and right neurons together ( left panel ) and separately; DAF-28::mCherry stereotypically accumulates in synapse-rich areas (ovals in A’ and B). Cell bodies of the ASI, ASJ, ASK, and likely Bag neurons are indicated. C. DAF-28::mCherry is secreted from mammalian cells. Dot blot of DAF-28::mCherry protein in media supernatant from transiently transfected HEK293T cells. The presence of DAF-28::mCherry protein in the media decreases dramatically following 24 hour incubation with 2.5 µg/mL BFA, indicating active vesicle-mediated release. Cell-number equivalents for the amount of media applied in each well are indicated at the bottom. D. Video of a section of the VNC (arrowheads) in the tail area, imaged from the ventral side. DAF-28::mCherry moves in bright puncta. A corresponding video file is Sup. Video 1 . Scale bar for B = 5μm, for D = 20μm.

Journal: bioRxiv

Article Title: IGF-like growth factors that couple food sensing to developmental decisions employ different release mechanisms in a single pair of C. elegans chemosensory neurons

doi: 10.64898/2026.01.23.701173

Figure Lengend Snippet: A. Confocal projection of the head of DAF-28::mCherry; pdaf-7 ::GFP animal, with both ASI neurons visible. DAF-28::mCherry protein (red) localizes exclusively to axons of the ASI and other neurons, and is excluded from dendrites. Green: GFP expressed from a pdaf-7 ::GFP; rol-6 array, used to visualize the ASI. The third dendrite in A is from another animal. A’ shows the axonal area. B. 3D confocal projections of axonal area of the animal in Figure 2B,C , showing both left and right neurons together ( left panel ) and separately; DAF-28::mCherry stereotypically accumulates in synapse-rich areas (ovals in A’ and B). Cell bodies of the ASI, ASJ, ASK, and likely Bag neurons are indicated. C. DAF-28::mCherry is secreted from mammalian cells. Dot blot of DAF-28::mCherry protein in media supernatant from transiently transfected HEK293T cells. The presence of DAF-28::mCherry protein in the media decreases dramatically following 24 hour incubation with 2.5 µg/mL BFA, indicating active vesicle-mediated release. Cell-number equivalents for the amount of media applied in each well are indicated at the bottom. D. Video of a section of the VNC (arrowheads) in the tail area, imaged from the ventral side. DAF-28::mCherry moves in bright puncta. A corresponding video file is Sup. Video 1 . Scale bar for B = 5μm, for D = 20μm.

Article Snippet: Cell supernatant was loaded into dot-blot apparatus (Bio-Rad) and transferred to nitrocellulose membrane (Bio-Rad).

Techniques: Dot Blot, Transfection, Incubation